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KMID : 0380020120270020097
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Korean Journal of Biotechnology and Bioengineering
2012 Volume.27 No. 2 p.97 ~ p.102
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Comparison of Biomass Productivity of Two Green Microalgae through Continuous Cultivation
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Gim Geun-Ho
Lee Young-Mi
Kim Duk-Jin
Jeong Sang-Hwa
Kim Si-Wouk
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Abstract
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In the present study, the biomass productivity of two green microalgae (Chlorella sp. and Dunaliella salina DCCBC2) were assessed in a 12 L tubular photobioreactor under optimum culture conditions. In the batch culture optimization process, the Chlorella sp. biomass was obtained as 1.2 g/L under atmospheric air as a sole CO2 source and other culture conditions as follows: light intensity, temperature, pH, NH4Cl and K2HPO4 were 100 μE/m2 /s, 27℃, 7.0, 20.0 mM and 2.0 mM, respectively. On the other hand, 2.9 g/L of D. salina DCCBC2 biomass production was observed under the following conditions: light intensity, temperature, pH, KNO3 and K2HPO4 were 80 μE/m2 /s, 27℃, 8.0, 3.0 mM and 0.025 mM, respectively. At 1% CO2 supply to the reactor, the Chlorella sp. production was reached 1.53 g/L with 25% increment under the same operating conditions. In addition, the maximum D. salina DCCBC2 biomass was observed as 3.40 g/L at 3% CO2 concentration. Based on the aforementioned optimized conditions, the dilution rate and maximal biomass productivity of Chlorella sp. and D. salina DCCBC2 in the continuous cultivation were 0.4/d and 0.6 g/L/d and 0.6/d and 1.5 g/L/d, respectively.
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KEYWORD
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green microalga, Chlorella, Dunaliella salina, photobioreactor, biomass productivity
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